Figure 3
Neutropenic IA results in extensive vascular and peribronchial invasion by A. fumigatus hyphae. (A) Extensive angioinvasion by A. fumigatus hyphae is observed in neutropenic mice but not in CGD or steroid-treated mice. The black arrows indicate the blood vessel at low magnification that was infiltrated by A. fumigatus hyphae and the white arrows indicate the A. fumigatus hyphae that infiltrate the vessel wall in neutropenic mice. CGD mouse lung images show a non-necrotizing granuloma in the lung parenchyma, with an unaffected adjacent blood vessel. Steroid-treated mouse lung images show phagocytic infiltration with intact adjacent blood vessel (left lower corner of lower panel). 200x (upper panels), 400x (lower panel in steroid-treated mice), 600x (lower panels for CGD and neutropenic mice). Two independent experiments were performed with similar results with a total of 4–6 mice per time-point. (B) Peribronchial localization of A. fumigatus hyphae is observed in neutropenic mice but not in CGD or steroid-treated mice. Magnification, 400x. Two independent experiments produced similar results with a total of 4–6 mice per time-point.
