Figure 4

Lineage-specific deletion of Cdc42 in Club cells and AECII in Kras mouse model. (a) Immunohistochemical staining for SP-C and CCSP in lung tumors from Kras and Kras/Cdc42 mice. The dashed line indicated the bronchiole. Br: Bronchiole. Scale bar = 50 μm. (b) Representative photos of CCSP-CreERT2/Rosa26R -LacZ (upper panel) or SPC-CreERT2/Rosa26R-LacZ (lower panel) mice lung sections at 4 weeks post tamoxifen administration. β-galactosidase staining (blue) indicates targeted cell. The upper panel is co-stained with CCSP and the bottom is co-stained with SP-C. Scale bar = 50 μm. (c) A brief scheme of mouse crossing of SPC-CreERT2 and CCSP-CreERT2 allele with Kras or Kras/Cdc42 mice. (d) Representative pathology and immunohistochemical staining for SP-C and CCSP of lung tumors derived from SPC-Cre-ERT2/Kras (SK), SPC-Cre-ERT2/Kras/Cdc42 (SKC), CCSP-Cre-ERT2/Kras (CK) and CCSP-Cre-ERT2/Kras /Cdc42 (CKC) mice at 8 weeks post Ad-Cre treatment. Br: Bronchiole; Al: Alveoli. Scale bar = 500 μm for H&E, Scale bar = 50 μm for immunostaining. (e) Statistical analyses of the number of alveolar and bronchiolar tumors found in SK and SKC mice. Data were shown as mean ± s.e.m. *P < 0.05. (f,g) Statistical analyses of the number of alveolar and bronchiolar tumors (f) and the percentage of bronchiolar tumors (g) found in CK and CKC mice. Data were shown as mean ± s.e.m. ***P < 0.001.