Figure 2

Sequence analysis of the MAT locus in Mucor irregularis. (A) Arrangement of MAT locus for the SexM (−) or SexP (+) alleles with their adjacent genes in M. irregularis. The SexM and SexP in M. irregularis are flanked by genes for RNA helicase and a triosephosphate transporter. The orientations of SexP and SexM genes are the same. The dissimilar DNA sequences of the sexM/P (from the TSS to stop codon) of M. irregularis are highlighted by a light grey box, gene orientation are show in the arrows. (B) Mating-type determination of M. irregularis isolates by PCR. DNA amplified with the MAT specific primer designed from5’ of tpt to the 5’ of RNA helicase. Lane 1: molecular ladder; Lane 2: M. irregularis CBS103.93; lane 3: no template control; lane 4: ddH₂O (as the negative control); lane 5: Rhizopus oryzae B81a (as the negative control); lanes e-t: M. irregularis isolates B50e- B50t. (C) Dot plot comparison between the homeodomain region of M. irregularis isolates CMFCCC B50k (x-axis) and CBS103.93 (y-axis).