Figure 3
From: Evolution of T Cell Responses during Measles Virus Infection and RNA Clearance
ELISPOT detection of IFN-γ and IL-17-secreting cells. PBMCs from MeV-infected macaques were cultured without in vitro stimulation (A,D) or were stimulated with H and N peptides (C) or MeV lysate (F) and cultured on plates coated with antibody to IFN-γ (A–C) or IL-17A (D–F) to determine numbers of spot-forming cells (SFCs) per 106 PBMCs. To determine the numbers of cells secreting IFN-γ (B) or IL-17 (E) in response to in vitro MeV stimulation, numbers of SFCs in unstimulated wells (A,D) were subtracted from the numbers of SFCs in MeV H and N (C) or MeV lysate (F)-stimulated wells. *P < 0.05; ***P < 0.001; ****P < 0.0001. One-way ANOVA with repeated measures followed by Bonferroni multiple comparisons test (n = 5).
