Figure 4 | Scientific Reports

Figure 4

From: RETRACTED ARTICLE: Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells

Figure 4The alternative text for this image may have been generated using AI.

The stability of eGFP expression in transfected CHO cells grown in the presence G418 selection pressure or absence of G418. (A) The stably transfected cells were passaged until 60 generation in the presence of G418 selection pressure (B) or in the absence of G418 selection pressure. The intensity of eGFP of cells were detected by flow cytometry at the passage 0, 10, 20, 30,40, 50, 60, respectively. The experiments were performed in triplicates. (C) The eGFP expression retention was calculated as the ratio of the MFI at the 60 generations stability testing to the MFI at the transient expression MFI testing. Retention of eGFP expression levels in cells transfected with CMV, CAG, CHEF-1α, CMV mutant, HEF1-α, mouse CMV, and CAG enhancer promoter-containing vectors (n = 3).

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