Figure 5 | Scientific Reports

Figure 5

From: RETRACTED ARTICLE: Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells

Figure 5

Gene copies per genome as determined by qPCR analysis. The gene copy number of CHO cells that stably transfected with CMV, CAG, CHEF-1α, HEF1-α element-containing vector was detected using qPCR at 10 generations post-transfection. (A) Thirty single stably clones was picked out and the gene copy number were analyzed by qPCR to detected the relationship between gene copy number. (B) The cells transfected with CMV promoter-containing vector was considered as 1.0. The relative copy number of the CAG, CHEF-1α, HEF1-α gene was calculated (*P < 0.05).

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