Table 1 Primer used in the analysis of gene copy number and mRNA expression levels.

From: RETRACTED ARTICLE: Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells

Primer designation

Type of analysis

Primer sequence (5′-3′)

EGFP-Fwd

qRT-PCR/qPCR

5′-CTACGTCCAGGAGCGCACCATCT-3′

EGFP-Rev

qRT-PCR/qPCR

5′-GTTCTTCTGCTTGTCGGCCATGATAT-3′

GAPDH-Fwd

qRT-PCR/qPCR

5′-CGACCCCTTCATTGACCTC-3′

GAPDH-Rev

qRT-PCR/qPCR

5′-CTCCACGACATACTCAGCACC-3′