Figure 1
From: SERPINB2 is a novel TGFβ-responsive lineage fate determinant of human bone marrow stromal cells
TGF-β1 promotes osteogenic differentiation. Human bone marrow stromal (skeletal) stem cells (hBMSC) were differentiated into osteoblasts (OS) using osteogenic induction mixture for 7 days. (A) Time line scheme of experimental setup illustrating TGF-β1 or SB-431542 (SB) treatment that was performed as either single pulse dose (TGFB1 1-dose or SB 1-dose) or continuous treatment (TGFB1 Contin. Or SB Contin.) at commitment and differentiation stages of in vitro OS differentiation (B) Mineralised calcium deposition was determined by Alizarin Red S staining, which is shown as microscopic images (20× magnification). (C) Alizarin Red Quantification under different experimental conditions: osteo-induced (OS), single dose of 10 ng/ml TGF-β1 (+TGFB1); continuous exposure to TGF-β1, TGFB1 con, SB 1-dose, and SB Con. Data are presented as the means ± SD of three independent experiments; n = 6; (D) qRT-PCR of ALPL (left panel) or RUNX2 (right panel) mRNA expression preformed on cells exposed to the indicated treatment on days 1, 3, and 7. Expression of each target gene was normalised to GAPDH. Data are presented as the means ± SD from three independent experiments, n = 6; *p < 0.05; **p < 0.01, ***p < 0.005.
