Figure 2
From: SERPINB2 is a novel TGFβ-responsive lineage fate determinant of human bone marrow stromal cells
TGF-β1 promotes adipogenic differentiation. (A) Time line schematic model illustrating the dose of TGF-β1 or SB-431542 (SB) treatment for either single pulse or continuous treatment at commitment and differentiation stages during adipogenic induction of human bone marrow stromal (skeletal) stem cells (hBMSC) (B) hBMSCs were induced into adipocyte using adipocyte induction medium in the presence of single dose (AD + TGFB1 1-dose) or continuous (AD + TGFB1 Contin.) exposure to 10 ng/ml TGF-β1 or a single (AD + SB 1-dose) or continuous (AD + SB Contin.) treatment with 10 µM SB-431542. Cells were stained on day 7 using oil red O staining for adipocytes containing lipid droplets, and shown as microscopic images (20×, magnification), non-induced (NI), adipocytic induced (AD). (C) Nile red quantification under the indicated treatment conditions was performed on day 7 post adipocyte induction. qRT-PCR quantification for LPL (D), PPARG2 (E), and ADIPOQ (F) mRNA under the indicated experimental conditions: non-induced (CNT), adipo-induced (AD), single dose of TGF-β1 (+TGFB1), continuous exposure to TGF-β1 (+TGFB1 Contin.), single dose of SB-431542 (+SB), and continuous dose of SB-431542 (+SB Contin.). Expression of each target gene was normalised to GAPDH. Data are presented as the means ± SD from three independent experiments, n = 6; *p < 0.05; **p < 0.01, ***p < 0.005.
