Table 3 Nucleotide pools in E. coli during stringent response. E. coli cultures were grown in defined minimal medium MOPS supplemented with 0.4% glucose at 37 °C with vigorous aeration until OD₆₀₀ 0.5. Then, stringent response was induced by mupirocin at 150 µg/ml (3 × MIC), and samples were withdrawn for nucleotide measurements at times indicated. The data are plotted as a function of time on (Fig. 5). 95% CI: 95% confidence interval. NA: not available.

Adenosines

ATP

79_[73;86]

83_[73;93]

85_[81;90]

86_[80;92]

87_[80;94]

86_[80;93]

82_[77;87]

ADP

19_[16;23]

13_[7.4;24]

12_[9.1;16]

11_[7.8;16]

10_[6.6;16]

13_[11;15]

15 _[13;18]

AMP

3.4_[2.7;4.3]

2.8_[1.2;6.7]

2.4_[1.7;3.4]

2.4_[1.3;4.5]

2.1_[1.0;4.1]

3.2_[1.9;5.2]

3.7_[2.8;4.8]

Guanosines

GTP

73 _[67;79]

48_[25;92]

30_[17;55]

25_[7.7;81]

23_[9.2;59]

33_[23;47]

32_[27;39]

GDP

20_[16;25]

10_[4.6;22]

5.4_[2.3;13]

3.6_[2.7;4.8]

3.6_[1.9;6.7]

5.4_[3.3;8.9]

6.2_[4.8;8.0]

GMP + IMP

4.4_[3.2;6.0]

3.8_[1.3;11]

2.3_[0.69;7.6]

1.7_[0.98;2.8]

2.1_[0.98;4.4]

3.4_[1.7;6.9]

3.4_[2.4;4.9]

ppGpp

1.4_[0.85;2.5]

27_[14;53]

52_[37;72]

60_[49;74]

60_[45;78]

50_[40;61]

47_[43;52]

pppGpp

NA

8.4_{[3.3; 21]}

8.7_{[3.5; 22]}

5.6_[0.38;81]

6.5_[2.0;21]

7.2_[4.4;12]

8.9_[7.5;10]

NTPs

CTP

11_[9.5;12]

14_[11;17]

16_[13;20]

18_[15;21]

20_[19;22]

21_[19;23]

23_[21;25]

UTP

14_[13;15]

15_[13;17]

16_[14;19]

17_[16;19]

19_[18;19]

20_[18;22]

23_[22;24]

ATP

52_[46;57]

60_[51;71]

60_[53;67]

57_[52;63]

53_[47;59]

51_[46;56]

44_[42;46]

GTP

26_[24;28]

15_[7.6;28]

10_[5.0;22]

9.8_[5.5;18]

10_[8.2;13]

10_[8.0;13]

11_[8.8;13]

AEC

ATP, ADP, AMP

87_[84;90]

90_[84;96]

91_[89;94]

92_[88;96]

92_[88;97]

90_[88;93]

88_[86;91]