Figure 3
From: SPI-2/CrmA inhibits IFN-β induction by targeting TBK1/IKKε
Knockdown of SPI-2 enhances VACV-triggered induction of IFN-β. (A) Effects of SPI-2-RNAi plasmids on the expression of SPI-2. HEK293 cells were transfected with the indicated plasmids for 24 hours before immunoblot analysis. (B) Effects of SPI-2-RNAi plasmids on the transcription of B13R and neighboring genes. SPI-2-RNAi stable THP-1 cells were infected with VACV strain WR for 12 hours before quantitative real-time PCR analysis. (C) Effects of SPI-2-RNAi plasmids on VACV-induced transcription of antiviral genes. SPI-2-RNAi stable THP-1 cells were infected with VACV strain WR for 12 hours before quantitative real-time PCR analysis. *p < 0.05, **p < 0.01, ***p < 0.001, relative to the control with VACV WR infection. (D,E) Effects of SPI-2-RNAi plasmids on HSV-1- and SeV-induced transcription of IFNB1. SPI-2-RNAi stable THP-1 cells were infected with HSV-1 (D) or SeV (E) for 12 hours before quantitative real-time PCR analysis.All experiments were repeated at least three times with similar results. The bar graphs show the mean ± S.D. (n = 3) of a representative experiment performed in triplicate. The cropped blots from different gels are displayed, for full blots refer supplementary information.
