Figure 4
From: SPI-2/CrmA inhibits IFN-β induction by targeting TBK1/IKKε
Effects of SPI-2 and CrmA on IFN-β promoter activation mediated by various components. (A,B) Effects of SPI-2 on IFN-β promoter (A) and ISRE reporter (B) activation mediated by cGAS, MITA, TBK1, IKKε, and IRF3. HEK293 cells were transfected with the IFN-β promoter or ISRE reporter and the indicated expression plasmids together with increasing amounts of SPI-2 for 20 hours before the luciferase reporter assays. (C) Effects of SPI-2 on IKKβ- and p65-mediated activation of IFN-β promoter. HEK293 cells (2 × 105) were transfected with the IFN-β promoter (0.1 μg) and IKKβ or p65 (0.1 μg each) together with increasing amounts of SPI-2 (25, 50, 100 ng) for 20 hours before the luciferase reporter assays. (D,E) Effects of CrmA on IFN-β promoter (D) and ISRE reporter (E) activation mediated by cGAS, MITA, TBK1, IKKε, and IRF3. The experiments were performed similarly to those in (A,B). (F) Effects of CrmA on IKKβ- and p65-mediated activation of IFN-β promoter. The experiments were performed similarly to those in (C). All experiments were repeated at least three times with similar results. The bar graphs show the mean ± S.D. (n = 3) of a representative experiment performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, relative to the controls transfected with the indicated components.
