Figure 6
a) Saccharification of EG (E5) and Xyn (X3) over-expressing lines of Arabidopsis and wild type plants (WT). Dried stem tissues (100 mg each) were ground into powder and incubated for 2 h at RT and then transferred to 80 °C (Xyn) and 90 °C (EG) for 20 h in a 5 ml reaction mixture containing optimum buffers for their activity. Reducing sugars in the hydroysates taken after 1, 2, 3, 4, 5, 10 and 20 h time intervals after centrifuged were measured by DNS assay using glucose as standard. (b) E5 and X3 transgenic lines were subjected to saccharification as above for 75 minutes with 1 °C increase in temperature after every 1 minute time period. The lysates taken after every 5 min were assayed for enzyme activity as well as release of sugars. The black line represents the temperature gradient depicting 1 °C increase in temperature after every 1 minute. Activity was expressed in nM of reducing sugars measured after the hydrolysis. The error bars represent standard deviation.
