Figure 2
From: Profiling of the metabolic transcriptome via single molecule molecular inversion probes
(a,b) IGV representation of the VHL locus of SKRC7 and SKRC7-VHLHA cells. BAM files containing whole RNAseq data from these cell lines were loaded into IGV. Note the CAA-UAA mutation, resulting in the VHL Q132-stop mutation at the protein level. c and d show SeqNext representations of the same VHL locus of SKRC7 (c) and SKRC7-VHLHA cells (d). (e) bar graph showing VHL-related TPM and FPM values of SKRC7 and SKRC7-VHLHA. (f) Western blot of SKRC7 cells and the VHL-expressing derivative, stained with an anti-HA antibody and an antibody against GAPDH as control house keeping protein. Panel f represents 2 cropped images from different western blots, loaded with the same protein samples, derived from SKRC7 and SKRC7-VHL cells as indicated. The corresponding full blots are presented in Supplementary Figure 2.
