Figure 1
From: Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device
Design, dimensions, and microchip assembly structure. The inner side of the bottom layer (BL) was produced printing the polyester film with one layer of toner creating a channel of 2.0 cm long and 0.2 cm wide and two circles of 0.3 cm of diameter at each end. The top layer (TL) has the same layout printed in the inner layer, but with the circles punched, BL and TL were treated with oxygen plasma and the three layers were laminated together. The channel height was defined by image printing with toner layer (~6 μm) applied over two polyester films that formed the TL, BL of the microchip, and an additional intermediary layer of polyester (middle layer - ML ~100 μm, which was cut through with a lases cutter). Due to the hot lamination step the microchannels height was approximately 103 μm as a result of the toner deformation. Female luers adapters were glued with epoxy resin on the channel end holes.
