Figure 4
From: Projection Stereolithographic Fabrication of BMP-2 Gene-activated Matrix for Bone Tissue Engineering
Assessment of osteogenic differentiation of hBMSCs encapsulated in gene-activated hydrogel. (A) ALP staining at day 14 post fabrication. Purple staining of ALP activity was only seen in the BMP group. Bar = 100 μm. (B) Real-time RT-PCR analysis of expression of osteogenic genes at day 28. All results were expressed relative to the level at day 0. RUNX2, Runt-related transcription factor 2; COL1, collagen type I; OCN, osteocalcin; and BSP2, bone sialoprotein II. Expression of all osteogenesis-associated genes was significantly higher in the BMP group compared to GFP group (**p < 0.01) (C) Osteocalcin immunohistochemistry. Brown staining of osteocalcin was detected only in the BMP group. Bar = 100 μm.
