Figure 1
From: The impact of wavelengths of LED light-therapy on endothelial cells
Experimental plan. Cells used for 2D experiments were stimulated on day 0 whereas cells embedded in 3D fibrin matrices were stimulation on day 0 and subsequently every 24 h until quantification was performed. HUVEC monolayers used for scratch assay were stimulated directly after performing the scratch and the migration was evaluated after 6 h. HUVEC for 2D proliferation experiments were stimulated after cells attached to the culture dish (approximately 2 hours after seeding) and counted every 24 h for 3 days. For 3D migration assays, NO measurement and angiogenesis protein array cells seeded to fibrin matrices were stimulated directly after polymerization of the fibrin clot and subsequently every 24 h for 4 days. Quantification of migrated cells was performed on day 4. 3D vascularization was determined by stimulating the cell-seeded fibrin clots directly after polymerization and every 24 h for one week. The quantification of 3D cell proliferation and vascularization was done after 4 and after 7 days.
