Figure 7 | Scientific Reports

Figure 7

From: CG200745, an HDAC inhibitor, induces anti-tumour effects in cholangiocarcinoma cell lines via miRNAs targeting the Hippo pathway

Figure 7

MiRNAs differentially expressed in response to CG200745 treatment and gene pathway analysis. (a) MiRNA expression profiles were analysed with a human Affymetrix GeneChip miRNA 4.0 Array and the expression induced by CG200745 treatment is shown in the heat map (upregulated, red). (b) Expression of individual miRNAs, as evaluated by qRT-PCR. *P ≤ 0.05, **P < 0.01, and ***P < 0.001. (c) SNU-1196 and SNU-1196/GR cells were transfected with miRNA mimics or a mimic control sequence and protein expression post-treatment (24 h) was analysed by western blotting. (d) Associations among highly ranked pathway-associated-genes (downregulated, green) and differentially expressed miRNAs (upregulated, red) were analysed following treatment with CG200745 (IC50). Solid and dotted lines indicate direct and indirect interactions, respectively. (e) SNU-1196 and SNU-1196/GR cells were transfected with miR-509-3p inhibitor or an inhibitor control sequence followed by CG200745 treatment and protein expression post-treatment (48 h) was analysed by western blotting.

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