Figure 3
Identification of the protein kinases involved in the Ser504 phosphorylation of eIF4B in neurons. (A–D), Representative western blot and quantification of phospho-Ser504 eIF4B (pS504-eIF4B) levels in primary hippocampal neurons treated for 3 h with 15 μM U0126, 20 nM rapamycin (Rapa) or 25 μM LY294002 (A,B) or pre-treated for 2 h with two CK inhibitors (10 μM D4476, 25 μM TBB) or a cPKC inhibitor (2 μM Gö6976; Gö), alone or in combination (C,D) and analyzed at rest (A,C) or after treatment with 10 μM bicuculline (Bic) for 30 min (B,D). Phosphorylated eIF4B levels are normalized against total eIF4B and shown as fold change over control (untreated neurons). Data are shown as mean ± SEM of at least three independent experiments for each treatment ((A,B) n = 3; (C,D) n = 4). Statistical significance is calculated using one-way ANOVA followed by Bonferroni post hoc test; *P < 0.05, **P < 0.01, ***P < 0.001.
