Table 4 Sequences of primers used in DNA cloning, dsRNA synthesis and qRT-PCR.
Primer name | Primer sequence (5′-3′) | Purpose |
|---|---|---|
TmELO1-F | CTGTGCGGAAAACGGAAGAG | cDNA cloning |
TmELO1-R | ATTTCGCCCCTGAGTTAC | |
TmELO2-F | AGTGACTTGTGTTCTGTG | |
TmELO2-R | AATTGCATTACGCCCGAGG | |
eTmELO1-F | CGGGATCCATGGCTCACCTAGTTACCAG | yeast expression |
eTmELO1-R | CCCTCGAGCTATTTGATCTTCTTTGAG | |
eTmELO2-F | CGGGATCCATGGCACAAATATTAAC | |
eTmELO2-R | CCCTCGAGTTACGCCCGAGGGTTGTCT | |
dsTmELO1-F1 | GATCACTAATACGACTCACTATAGGGGTGTCCAAACACTGTTCAG | dsRNA fragments |
dsTmELO1-R1 | GATCACTAATACGACTCACTATAGGGATTGCCGCCACCATGTAA | |
dsTmELO2-F1 | GATCACTAATACGACTCACTATAGGGCACGACCTCATGGATAAC | |
dsTmELO2-R1 | GATCACTAATACGACTCACTATAGGGTGGAGGGTCGAAATGTGG | |
dsTmELO1-F2 | GATCACTAATACGACTCACTATAGGGATGGCTCACCTAGTTACCAG | |
dsTmELO1-R2 | GATCACTAATACGACTCACTATAGGGGTTGACACCTGAAGCTGT | |
dsTmELO2-F2 | GATCACTAATACGACTCACTATAGGGCTAGATACGATCTTCTTCG | |
dsTmELO2-R2 | GATCACTAATACGACTCACTATAGGGTAGACCTATCCACCAGAC | |
dsEGFP-F | GATCACTAATACGACTCACTATAGGGATGGTGAGCAAGGGCGAGGAGC | |
dsEGFP-R | GATCACTAATACGACTCACTATAGGGGATCACTAATACGACTCACTATAGGG | |
qTmELO1-F | TACTCATCAGTTCCAGCTTCT | qRT-PCR |
qTmELO1-R | CACCGTTATTACTACTCTTCACA | |
qTmELO2-F | CACGCCGTCATGTTCTACT | |
qTmELO2-R | ACGCCCGAGGGTTGTCTAT | |
qTmRpS3-F | GTGGTCGTTTCTGGCAAACT | |
qTmRpS3-R | CAACACTCCTTGCCTCAACA |
