Figure 3 | Scientific Reports

Figure 3

From: Oxygen Mapping of Melanoma Spheroids using Small Molecule Platinum Probe and Phosphorescence Lifetime Imaging Microscopy

Figure 3

Lifetime distribution of PtLsCl (100 µM, 12 hours) in melanoma MCTS using one-photon PLIM. PtLsCl lifetime distribution across spheroid represented as: (A) Continuous rainbow scale, range: 0–11.5 µs. (B) Discrete colours, reveals three distinct spheroid regions: an outer proliferative rim (red, 0–3.0 µs), an intermediate necrotic region (green, 3.0–7.5 µs) and an inner necrotic core (blue, 7.5–12.0 µs). (Magnification 40x scale bar = 100 µm). (C) Emission decay observed in inner (a), middle (b) and outer (a) area of spheroid showed short-lived emission in outer area and a longer emission in inner area.

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