Table 1 Altered LM profiles of diseased tendon stromal cells when compared to those from healthy tendon stromal cells.

From: Increased 15-PGDH expression leads to dysregulated resolution responses in stromal cells from patients with chronic tendinopathy

DHA bioactive metabolome

Q1

Q3

Tendon stromal cells Lipid mediators levels (pg/incubation)

Healthy + IL-1β

Disease + IL-1β

RvD1

375

141

5.3 ± 1.8

4.6 ± 2.0

RvD2

375

141

8.7 ± 4.3

11.6 ± 5.3

RvD3

375

147

4.1 ± 2.5

6.6 ± 2.7

RvD4

375

101

4.2 ± 2.1

7.9 ± 3.3

RvD5

359

199

53.9 ± 11.1

49.3 ± 7.1

RvD6

359

101

24.1 ± 7.7

13.9 ± 2.7

17R-RvD1

375

141

1.9 ± 0.6

2.1 ± 0.6

17R-RvD3

375

147

5.7 ± 1.7

7.6 ± 2.1

PD1

359

153

27.6 ± 5.8

26.6 ± 9.5

17R-PD1

359

153

1.0 ± 0.2

1.7 ± 1.0

10 S,17S-diHDHA

359

153

139.7 ± 10.3

117.1 ± 41.3

MaR1

359

221

6.3 ± 2.4

28.7 ± 11.8*

7 S,14S-diHDHA

359

221

40.5 ± 22.3

7.9 ± 2.9

4 S,14S-diHDHA

359

101

15.8 ± 6.8

19.8 ± 6.9

n-3 DPA bioactive metabolome

RvD1n-3 DPA

377

143

2.0 ± 0.8

4.8 ± 1.0*

RvD2n-3 DPA

377

261

0.7 ± 0.6

2.3 ± 1.3

RvD5n-3 DPA

361

263

19.9 ± 4.3

23.0 ± 5.9

PD1n-3 DPA

361

183

58.0 ± 26.4

98.6 ± 54.0

10 S,17S-diHDPA

361

183

414.3 ± 207.1

499.3 ± 263.5

MaR1n-3 DPA

361

249

13.8 ± 5.6

13.3 ± 4.5

EPA bioactive metabolome

RvE1

349

195

0.3 ± 0.2

0.1 ± 0.1

RvE2

333

199

39.8 ± 10.1

54.3 ± 6.0

RvE3

333

201

2.0 ± 1.1

2.0 ± 0.9

AA bioactive metabolome

LXA4

351

217

2.2 ± 1.0

6.0 ± 1.7*

LXB4

351

221

40.9 ± 18.5

96.2 ± 52.9

5 S,15S-diHETE

335

235

1404.0 ± 358.7

1727.2 ± 437.5

15epi-LXA4

351

217

82.8 ± 48.4

245.2 ± 78.3*

15epi-LXB4

351

221

57.7 ± 16.8

43.6 ± 12.4

15-oxo-LXA4

349

233

0.5 ± 0.2

3.0 ± 1.3*

LTB4

335

195

57.0 ± 49.4

0.0 ± 0.0

5 S,12S-diHETE

335

195

138.8 ± 63.9

264.8 ± 88.0

PGD2

351

189

2065.8 ± 906.4

968.0 ± 294.1

PGE2

351

189

9483.0 ± 4429.1

21371.2 ± 8192.9

PGF2α

353

193

367.0 ± 79.8

380.1 ± 102.5

TxB2

369

169

20.8 ± 5.8

12.1 ± 2.2

  1. Tendon stromal cells (60,000 cells per well) were derived from healthy hamstring (n = 8 donors) or diseased supraspinatus tendons (n = 8 donors). Cells were incubated for 24 h with IL-1β, incubations were quenched using ice-cold methanol containing deuterium labelled internal standards and lipid mediators (LM) were identified and quantified using LM profiling (see methods for details). Q1, M-H (parent ion) and Q3, diagnostic ion in the MS-MS (daughter ion). Results are expressed as pg/incubation. Mean ± SEM of n = 8 per incubation. *P < 0.05 vs Healthy. The detection limit was ~ 0.1 pg. -, Below levels found in media alone.
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