Figure 3 | Scientific Reports

Figure 3

From: Integrated Method to Attach DNA Handles and Functionally Select Proteins to Study Folding and Protein-Ligand Interactions with Optical Tweezers

Figure 3

Single molecule experiments with the CBD-A. (a) Schematic representation of the optical tweezers experimental set-up. (b) Representative force-extension curves of the CBD-A in the unliganded state. Protein samples were purified by functional selection (top panel) and by electro-elution (bottom panel). (c) Change in extension upon unfolding vs. force for functionally selected (blue) and electro-eluted (orange) samples. The gray dashed line shows the theoretical Worm Like Chain (WLC) model of the unfolded CBD-A, using a contour length of 46.5 nm and a folded distance of 2 nm.

Back to article page