Figure 4 | Scientific Reports

Figure 4

From: Integrated Method to Attach DNA Handles and Functionally Select Proteins to Study Folding and Protein-Ligand Interactions with Optical Tweezers

Figure 4

Kinetic analysis of CBD-A and cAMP interactions. (a) Representative force-extension curve of the CBD-A in the absence (left) and in the presence of [cAMP] = 0.5 mM (right). (b) Unfolding force probability distribution for the CBD-A in the absence (light blue, N = 1157) and in the presence of cAMP (dark blue, N = 829). The solid line represents the unfolding force distribution reconstructed from the force-dependent lifetimes (see main text). (c) Folded state lifetimes (τ folded) as a function of force extracted from the unfolding force distributions in the absence (light blue squares) and presence of cAMP (dark blue squares). The dashed line corresponds to the fit of Bell’s model to the force-dependent rates.

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