Figure 3 | Scientific Reports

Figure 3

From: Dissecting the link between the enzymatic activity and the SaPI inducing capacity of the phage 80α dUTPase

Figure 3

Central channel reorganization induced by D95E mutation. Structural superimposition of trimeric Dut80α wt and D95E structures (coloured as Fig. 2) shows that D95E mutation induces small structural changes in the trimer central channel restricted to the residue 95 side-chain reorientation. Two orthogonal close-views of the central channel around position 95 are shown with these residues as sticks. The disposition of Glu95 in Type I crystal impairs the coordination of the Mg ion in the central channel and this position is occupied by a water molecule (grey sphere labelled as W-Type I). However, in Type II crystals Glu95 side-chains acquire an alternative conformation that allows Mg ion coordination (magenta sphere labelled as Mg-Type II) but displaced with respect to the Mg ion observed in the wt structure (magenta sphere labelled as Mg-WT).

Back to article page