Figure 4

Extinction memory requires de novo protein synthesis after its reactivation in order to persist. (A) Schematic representation of the experimental procedures. Fear conditioned rats were submitted to 30-min extinction session or remained in their homecages. A reactivation session was conducted 7 days later followed by a test in the next day. Immediately after reactivation, animals received i.p. infusion of cycloheximide (Extinction group: N = 10; No-extinction group: N = 14) or its vehicle (Extinction group: N = 9; No-extinction group: N = 11). (B) Freezing levels during extinction session. (C) Freezing levels during reactivation session. (D) Freezing levels during test session. (*) Significant differences between groups (P < 0.05, Repeated-Measures or Two-Way ANOVA followed by Tukey’s post-hoc test).