Figure 2
Implementation of the motion correction algorithm in RSOM100. (a) Skin surface detected in the raw data before and after motion correction. (b) Vertical MAPs of a region of interest on the lower arm surrounding a benign nevus, before and after motion correction. The labels “(d)” and “(e)” mark the location of the epidermal-dermal junction and the dermal layer shown as lateral MAPs in panels d and e respectively. (c) Mean CNR in the cross-sectional MAPs in panel b as a function of depth before and after motion correction. Reconstructions were based on the frequency bands 20–60 MHz (left) or 60–180 MHz (right). (d) Lateral MAP of the epidermal-dermal junction before and after motion correction. The insets are zoomed-in views of the areas enclosed in white dashed boxes, showing single capillary loops after motion correction. Magenta boxes enclose areas shown as zoomed-in views in panel f. (e) Lateral MAP of an extensive vascular network in the dermal layer, showing well-resolved microvessels (green) after motion correction. (f) Zoomed-in views of the regions boxed in magenta in panel d. White dashed lines trace the lateral profile in panel g. (g) Lateral profile through four distinct capillary loops. Peak FWHM values are shown. Scale bar, 500 µm. Abbreviations: d: depth; fs: fast-scanning; ss, slow-scanning.
