Figure 5
From: Neuropilin-1 is upregulated by Wnt/β-catenin signaling and is important for mammary stem cells
Loss of Nrp1 in MaSCs severely delays mammary development. (A) Experimental setup for Nrp1 conditional knockout using Procr-CreERT2 line. TAM was administrated at 3-week at every other day for a total of three times, mammary glands were harvest at 7-week. (B) Whole mount carmine staining of 7-week old Procr-CreERT2, Procr-CreERT2;Nrp1 fl/+ and Procr-CreERT2;Nrp1 fl/fl mammary glands. Dash line, epithelial front; dash line circle, LN. Scale bars, 2 mm. (C) Quantification of the mammary duct extension in Procr-CreERT2 (+/+), Procr-CreERT2;Nrp1 fl/+ (fl/+) and Procr-CreERT2;Nrp1 fl/fl (fl/fl) mice. The distance from L.N. to epithelium forefront was measured. n = 4 mice in each group. Data are presented as mean ± SEM. ***P < 0.0001. (D) qPCR analysis validating the Nrp1 knockout efficiency in FACS-isolated basal cells. Data are presented as mean ± SEM. ***P < 0.0001.
