Figure 6

Circadian expression of chemoattractants after L. major infection in vivo. (A,B) Mip2, Mcp1, Mip1α, Mip1β and Tnfα expression in peritoneal exudate cells (PECs) of L. major-infected or PBS-injected mice 3 h (A) or 6 h (B) (n = 5) after injection. Significant rhythms are illustrated with fitted cosine curves, otherwise data are simply connected by straight lines between data points, indicating no significant cosine fit. (C) Cytokine/chemokine expression 6 h after L. major or PBS injection in chimeric mice consisting of irradiated B6.SJL hosts that have received bone marrow cells from either Bmal1 ^+/+ OT-1 Rag2 ^−/− (WT-BM) or Bmal1 ^−/− OT-1 Rag2 ^−/− donors (KO-BM). In A and B, data are presented as mean ± SEM; Significant rhythms are illustrated with fitted cosine curves, otherwise data are simply connected by straight lines between data points, indicating no significant cosine fit; significance between the two groups are indicated by stars: *p < 0.05; **p < 0.01; ***p < 0.001. Significant differences between are indicated by stars: *p < 0.05; **p < 0.01, ***p < 0.001; different letters indicate significant differences between time points within the L. major group. In (C), significant differences between the time points are indicated by stars: *p < 0.05; **p < 0.01. For details of statistics, see Supplementary Table S3.