Figure 3 | Scientific Reports

Figure 3

From: Creation and Initial Characterization of Isogenic Helicobacter pylori CagA EPIYA Variants Reveals Differential Activation of Host Cell Signaling Pathways

Figure 3

Presence of an EPIYA-D motif induces the greatest IL-8 secretion. AGS cells were infected with the isogenic strains at an MOI of 10. A sample of the co-culture supernatant was taken at 12, 24, and 36 hrs post-infection and analyzed by ELISA for total IL-8. At all time points, there was a significant increase in IL-8 secretion in cells infected with H. pylori (P = 0.0001 for all comparisons, two-way ANOVA). At 24 and 36 hrs, there was a significant increase in IL-8 secretion from cells infected with H. pylori expressing CagA with an intact EPIYA motif as compared to the truncated CagA protein (P = 0.0001, two-way ANOVA). There was no difference in secretion from cells infected with increasing EPIYA-C motifs. *Indicates significance compared to EPIYA-ABTD at 24 hrs; ^Indicates significance compared to EPIYA-ABTD at 36 hrs. ELISA data are presented as the geometric mean +95% confidence interval and represent three independent experiments.

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