Figure 4 | Scientific Reports

Figure 4

From: Creation and Initial Characterization of Isogenic Helicobacter pylori CagA EPIYA Variants Reveals Differential Activation of Host Cell Signaling Pathways

Figure 4

CagA EPIYA polymorphism effects on CagA translocation, phosphorylation, and activation of ERK1/2. AGS cells were infected with the isogenic strains at an MOI of 100 and cells were harvested in a temporal fashion. Lysates were then analyzed for (a and b) phosphorylated CagA (pTyr), (c) total CagA (CagA), (d) total ERK1/2 (ERK1/2) and (e and f) phosphorylated ERK1/2 (pERK1/2). Data were normalized to GAPDH and the value at time zero for each strain for each protein of interest was designated as “1” and variation from that value were then plotted temporally. The data are displayed as the geometric mean of four biologically independent experiments.

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