Figure 2

Analysis of Microbial Mock Community HM-783D. (A) Rarefaction curves of sequencing data obtained using mock microbial community HM-783D for both V1V2 and V4V5 regions of the 16S rRNA gene. Sequences were subsampled in increments of 500 to an even depth of 14,000 sequences per sample. (B) Shannon and (C) Simpson diversity calculated for the mock community using the V1V2 and V4V5 hypervariable regions. (D) Theoretical and observed relative abundances of genera detected in HM-783D using the V1V2 and V4V5 hypervariable regions.