Table 2 Oligonucleotide sequences for primers used in qRT-PCR.
Reference genes | Forward primer | Reverse primer |
|---|---|---|
Target genes | Forward primer | Reverse primer |
α-tubulin1A | 5′-TGACAATGAGGCCATCTATG-3′ | 5′-TGCTTCCATACCCAGGAATGA-3′ |
CG13220 | 5′-TGTTCAGTTTTGGCTCTGTTCTGA-3′ | 5′-ACTGTTCTCCGGCAGAATGC-3′ |
Ubi | 5′-GACTTTGAGGTGTGGCGTAG-3′ | 5′-GGATCACAAACACAGAACGA-3′ |
GAPDH | 5′-GTCTGATGACAACAGTGCAT-3′ | 5′-GTCCATCACGCCACAACTTTC-3′ |
RP49 | 5′-CGCTACAAGAAGCTTAAGAGGTCAT-3′ | 5′-CCTACGGCGCACTCTGTTG-3′ |
β-actin | 5′-AATTACCATTGGTAACGAGCGATT-3′ | 5′-TGCTTCCATACCCAGGAATGA-3′ |
EF1α | 5′-GATGCTCCAGGCCACAGAGA-3′ | 5′-TGCACAGTCGGCCTGTGAT-3′ |
SgVKR | 5′-GCATCTTGGCATTGATTTGCTA-3′ | 5′-GGAATCTCCCATTTGTCAAGAGTT-3′ |
SgSpo | 5′-CAACATCTTCACCAGCTACATGTG-3′ | 5′-GGGTCGTCGTAGTCGAAGGA-3′ |
SgPhm | 5′-CGCAGAGCCCGGACAAC-3′ | 5′-CGAACATGTCGGCCATGA-3′ |
SgDib | 5′-TAGCTGGAATGGACACAACATC-3′ | 5′-CTGGGTCTGGAAAATACTCTGG-3′ |
SgSad | 5′-ATCGTGGCCGAGATTACGAA-3′ | 5′-AGCACCATCTCCGGATCCT-3′ |
SgShd | 5′-CCGCCGTCATTGACTTCATA-3′ | 5′-GTGAGCTCCCAAGCGTGG-3′ |
SgVg1 | 5′-CCGCTGAACATCACTGCAAT-3′ | 5′-ACTTGGGCCAAATGGATGAG-3′ |
SgVg2 | 5′-GCTACCCGCAATCTGTAAAATACA-3′ | 5′-CGACTGTGAAAGGGCATTGA-3′ |
SgKr-h1 | 5′-CTCCAAGACGTTCATCCAGAG-3′ | 5′-TGCTTGGAGCAGGTGAAG-3′ |
SgJHAMT | 5′-CGGAGCAAAGGCAAGCA-3′ | 5′-CCACTTCACCGCCTGGTTT-3′ |
SgCYP15A1 | 5′-AAAGCAACTTCATCATTCACAGATG-3′ | 5′-CAGAGCCAGCCATGAACAAA-3′ |
