Figure 6
Evaluation of conditioned medium of Streptococcus salivarius strains on gingival re-epithelialization. (a) Re-epithelialization parameters (μm, A, and μm*A) of the S. salivarius conditioned medium relative to the TSB control. (b) Re-epithelialization performance values obtained with desalted-conditioned medium (7 kDa cut-off) with or without proteinase K treatment (200 μg/ml, 1 hour at 37 °C). S. salivarius MS-oral-D6 desalted-conditioned medium after PMSF treatment (1 mM, 1 hour at room temperature). Results are expressed as mean ± SEM from two independent experiments performed in triplicates. Significant differences from the non-treated control were assessed by a one-way ANOVA using a Dunnett’s test for multiple comparisons (*P < 0.05; **P < 0.001; ***P < 0.0001). (c) Silver staining of the proteins present in desalted-conditioned medium before and after proteinase K (Prot. K) treatment. Arrow indicates the high MW band detected in S. salivarius MS-oral-D6 conditioned medium. (d) Abundant proteins identified in the high MW band of the S. salivarius MS-oral-D6 conditioned medium. The bar labelled as “other” represents the sum abundance of seven other identified secreted proteins. Intensity based absolute quantitation (iBAQ) refers to the total intensity corrected for the number of measurable peptides within a sample.
