Table 1 Cell screening of pBAC-hLF transfection.

From: Large-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows

Cell line

Culture method

Isolated colonies

PCR positive cell colonies

Freezing cell colonies

Cell colonies for NT

0901FFB

Single cell culture

375

5

5

#18, #22, #35

0904FFB

Single cell culture

577

7

7

#4

1003FFB

Single cell culture

315

4

4

#17, #20