Figure 3
From: Overlapping and Specific Functions of the Hsp104 N Domain Define Its Role in Protein Disaggregation
Direct binding of Hsp104N to the C1 peptide determined by biolayer interferometry. (a) Binding of Hsp104N (solid lines) or BSA (dotted line) to immobilized biotinylated C1 peptide was measured by light distance shift (nm). A representative curve set of association (0–10 min) and dissociation (10–20 min) curves for various Hsp104N concentrations (4.3 to 137 µM) or BSA control (250 µM) from two independent measurements are shown. (b) Averaged equilibrium light distance shift at different Hsp104N protein concentrations from two independent measurements.
