Figure 5
Expression profile of CaPAP7 in genotypes differing for seed weight and phytate content. (A) Expression pattern of CaPAP7 in young pod in contrasting chickpea accessions. Fold change was calculated using EF1-α as reference gene. Three biological replicates were considered for expression analysis. Each biological replicate comprise of pooled sample from multiple plants. Error bars indicate SE (n = 3). (B) Phytic acid content of contrasting chickpea accessions (Joshi-Saha et al.33). Error bars represent SE (n = 2) (C) Average seed weight (100 seed weight in g) of contrasting chickpea accessions (Kujur et al.32). Error bars represent SE (n = 3) CaPAP7 expression was in negative correlation with the seed weight (r = −0.82) and seed phytate content (r = −0.99). Different letters at the top of bar indicates different significant classes determined by one-way ANOVA followed by Duncan’s multiple range test.
