Figure 7
Effect of purified recombinant CaPAP7 on phytate hydrolysis and Arabidopsis growth parameters. (A) Phenotypic deference in P supplemented ( + P), P deficient (−P), P deficient + phytate (phytate) and P deficient + phytate + CaPAP7 (phytate + E) grown Arabidopsis seedlings. (B) Visualization of acid phosphatase activity by BCIP overlay on roots of Arabidopsis seedlings. (C) Average root length (n = 7 in six replicates). (D) Average lateral root length (n = 7 in six replicates). (E) Average biomass per plant (n = 7 in six replicates). (F) Total soluble Pi (nmoles per plant) (n = 6 in six replicates). All analysis was done after 7 days of growth on above said media. Error bars represent SE. Students t-test was performed for the statistical analysis **p < 0.001, *p < 0.05.
