Figure 1
From: Impact of mitochondrial nitrite reductase on hemodynamics and myocardial contractility
(A–D) Experimental design and measurement of hematocrit (Hct), arterial oxygen partial pressure (pO2) and arterial oxygen saturation (sO2) over time. In vivo experiments were performed in three groups: with normal Hct under (a) normoxic (Hct40-NOX: blue bars) and (b) hypoxic (Hct40-HOX: red bars) conditions and (c) halved Hct under hypoxic (Hct20-HOX: green bars) conditions. Experimental design includes anesthesia and catheter placement for Hct adjustment (HCT ADJ), pO2 adjustment (NOX: normoxic group 100 mmHg; HOX: hypoxic groups: 50 mmHg), hemodynamic monitoring and blood sampling. A bolus of nitrite (15 µmol/kg) was infused intra venously after pO2 adjustment. Blood samples were taken at baseline (BL), after HCT ADJ, after NOX/HOX adjustment, 10 and 20 minutes after nitrite infusion (A). Changes on Hct (B), pO2 (C) and sO2 (D) values over time in all three groups determined by blood gas analysis. Data are expressed as mean ± SEM of at least n = 8, ***p < 0.001. One animal died upon adjustment of Hct.
