Figure 3
This figure exemplarily shows the process of automated SD detection. Panel (a) shows a segment of a fluorescence-wide field image, which was 3D-SIM-reconstructed (b). As shown in the graph in panel (b), dFP was measured as the peak-to-peak distance of neighboring SD. For an automatized approach, areas of plan view capillary sections were selected and automatic SD detection was performed. The red lines in panel (c) show the segmented SD, the length that was measured by the FIJI plugin. The scale bar indicates 2 µm.
