Figure 7 | Scientific Reports

Figure 7

From: Structured illumination microscopy and automatized image processing as a rapid diagnostic tool for podocyte effacement

Figure 7

Picture (a) shows a view of the surface of glomerular capillaries labeled with nephrin in a single frame of a wide field microscopy z-stack. The image stack was processed by deconvolution enhancing the details of the interdigitating foot processes in picture (b). Picture (c) shows, that the highest resolution could be accomplished by SIM reconstruction. Pictures (d,e and f) show the results of SD detection using our plugin (red lines) for wide field (d), deconvoluted wide field (e) and 3D-SIM (f). The scale bars indicate 10 µm in (c) and 1 µm in (f).

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