Figure 8
The scheme in (a) exemplarily shows the geometric bias of TEM measurement. In TEM, measurement of dFP will lead to false high values (I) compared to when foot processes are measured in an exactly orthogonal section (II). The graph in (b) shows the mean values of dFP measured in the same patient by TEM and SIM. SIM shows a significantly smaller dFP and StdDev compared to the measurements taken in the TEM pictures (p < 0.001, Student’s t test). Picture (c) shows the ultrastructural aspect of an MCD patient biopsy. Podocyte FP are effaced and significantly wider (red double arrows in c and d) compared to FP of the same patient shown in the SIM data in (d). The asterisks mark the capillary lumen, RBC indicates two adjacent red blood cells and the arrowheads indicate the position of effaced FP. The scale bars indicate 2 µm in the upper and 1 µm in the lower panel.
