Figure 1
From: Ultraviolet Radiation-Induced Production of Nitric Oxide:A multi-cell and multi-donor analysis
Assessment of UV-A and UV-B’s relative ability to damage DNA. (a) γ-H2AX western blot of lysates from primary FSKs irradiated with No UV (No), 9 J/cm2 UV-A (A), 0.09 J/cm2 UV-B (B) or both 9 J/cm2 UV-A and 0.09 J/cm2 UV-B (A + B) harvested at either 0 or 5 hours (0 or 5) post-irradiation. Densitometry of γ-H2AX levels, normalised to actin, is given showing the relative intensity of the bands. (Images shown here are cropped from larger scanned images. Complete gel scans are included as Supplementary Figure 1). (b) 6-4PP formation in DNA from primary FSKs isolated from three donor skins immediately following exposure to No UV (No), 9 J/cm2 UV-A (A), 0.09 J/cm2 UV-B (B) or both 9 J/cm2 UV-A and 0.09 J/cm2 UV-B (A + B). (Error bars are standard deviations of duplicate samples).
