Figure 1

Solubilisation of DMPC vesicles by SMA(2:1) at 30 °C as monitored by ³¹P NMR. (A) NMR spectra of 10 mM DMPC initially present in the form of LUVs upon exposure to increasing concentrations of SMA(2:1). (B) Peak areas, A, at four different DMPC concentrations as functions of SMA(2:1) concentration, showing experimental data (circles) and global fits (lines) according to equations 9–11. The slight increase in A upon complete solubilisation is due to a decrease in nanodisc size with increasing SMA(2:1) concentration, thus resulting in sharper peaks that are better resolved from the baseline, as seen in A. (C) Phase diagram of DMPC/SMA(2:1) at 30 °C showing the onset (saturation; SAT) and completion (solubilisation; SOL) of solubilisation. Shown are pairs of \({c}_{{\rm{S}}}^{{\rm{SAT}}}\,\)and \({c}_{{\rm{S}}}^{{\rm{SOL}}}\) (circles) obtained from breakpoints derived from local fits in (B) and global fits (solid lines).