Table 2 List of primers for amplifying target genes.
Gene | Primer name | Primer sequence | Restriction enzyme |
|---|---|---|---|
daf-16 | daf-16-F | 5′ AAAACTGCAGAGTACAGCAATTCCCAAATGAAA 3′ | Pst I |
daf-16-R | 5′ CCCAAGCTTAATTGGATTTCGAAGAAGTGGAT 3′ | Hind III | |
skn-1 | skn-1-F | 5′ AAAACTGCAGGGGTACCCACTTGCCCTATT 3′ | Pst I |
skn-1-R | 5′CCCAAGCTTTCATTTCAGCCACTCACTGC 3′ | Hind III | |
hsf-1 | hsf-1-F | 5′AAAACTGCAGGAAAAAAAGTAGGAGCAAAAAAT3′ | Pst I |
hsf-1-R | 5′CGGGGTACCAGTCAAAAAGCTGAAAAAATCGG3′ | Kpn I | |
hsp16.2 | hsp16.2-F | 5′ CGGGGTACC ATTCAGCAGATTTCTCTTCGACGATT 3′ | Kpn I |
hsp16.2-R | 5′ CCGCTCGAGTGTCACTTTACCACTATTTCCGTCC 3′ | Xho I | |
sod-3 | sod-3-F | 5′ CGGGGTACCAGCTCCTTTTAAATTAAGACA 3′ | Kpn I |
sod-3-R | 5′ CCGCTCGAGTATTCTTCCAGTTGGCAAT 3′ | Xho I |