Figure 1

VEGF expression was positively correlated with CRT expression and differentiated histology in human NB tumors. (A) CRT and VEGF mRNA expressions in 56 human NB tumors were determined by real-time PCR and normalized to the internal control GAPDH. The correlation between expression levels of CRT (x axis) and VEGF (y axis) was analyzed by Spearman’s correlation test (Spearman’s ρ = 0.648, P < 0.001). (B) CRT and VEGF mRNA levels in 31 NB tumors were evaluated by real-time PCR. The levels of CRT (shaded bar) and VEGF (solid bar) are higher in differentiated NB (DNB) than in undifferentiated NB (UNB). (C) The CRT mRNA expression was determined by real-time PCR in 56 human NB tumors and presented as the mean ± SEM of each group, undifferentiated NB (N = 27) versus differentiated NB (N = 29). The CRT mRNA expression was significantly increased in differentiated NB. (D) The VEGF mRNA expression was determined by real-time PCR in 56 human NB tumors and presented as the mean ± SEM of each group, UNB (N = 27) versus DNB (N = 29). The VEGF mRNA expression was significantly increased in differentiated NB. **P < 0.01, ***P < 0.005.