Figure 3
From: Impact of protein O-GlcNAcylation on neural tube malformation in diabetic embryopathy
Activation of OGT in the neural tube of embryos from diabetic mice. (A) Immunoblot assay of phosphorylated tyrosine residues (p-Tyr) in immunoprecipitated (IP) OGT and total OGT in tissue lysates (input). β-actin, loading control. (B) Quantification of the immunoblot bands. Data are presented as mean ± SD. p = 0.0093; n = 3 embryos from 3 litters. DM, diabetic; ND, non-diabetic. Full-length blots are included in the Supplementary Information (Figure S2).
