Figure 3

Enhanced proliferative activity of Mac-ASCs. (A) A total of 1000 cells (Sat- or Mac-ASCs) were seeded in a 96-well plate, and the MTT assay was performed at different time points. The growth curves of the two cell lines showed significant differences (P < 0.001). (B and C) Sat- (B) and Mac- (C) ASCs were stained with PI and analyzed by flow cytometry for cell cycle. Pink, gridded, and blue shaded peaks represent the G0/G1, S, and G2/M phases, respectively. (D) Quantification of cell proportions in the G0/G1, S, and G2/M phases in Sat- and Mac-ASCs. (E and F) Sat- (E) and Mac- (F) ASCs were co-stained with Annexin V and PI, and analyzed by flow cytometry for cell apoptosis. No difference was observed between Mac-ASCs and Sat-ASCs.