Figure 4

Altered multi-lineage differentiation potential of Mac-ASCs. (A–D) Uninduced Sat- (A) and Mac- (B) ASCs, or adipogenic-induced Sat- (C) and Mac- (D) ASCs were stained with Oil Red. Lipid droplets were stained by Oil Red O, and appeared orange in the cytosol. Scale bars, 50 μm. (E) qRT-PCR analysis of the adipogenic genes Adiponectin, C/EBPα and PPARγ in adipogenic-induced Sat- and Mac-ASCs. (F–I) Uninduced Sat- (F) and Mac- (G) ASCs, or osteogenic-induced Sat- (H) and Mac- (I) ASCs were stained with Alizarin Red. Bone nodules were stained by Alizarin Red and appeared red in culture. Scale bars, 100 μm. (J) qRT-PCR analysis of the osteogenic genes AKP, Osteocalcin and RUNX2 in osteogenic-induced Sat- and Mac-ASCs. (K–N) Uninduced Sat- (K) and Mac- (L) ASCs, or chondrogenic-induced Sat- (M) and Mac- (N) ASCs were stained with antibody against Collagen II and counterstained with hematoxylin. Collagen II signals appeared brown in the cytosol. Scale bars, 50 μm. (O) qRT-PCR analysis of the chondrogenic genes Aggrecan, Sox9 and Col2A1 in chondrogenic-induced Sat- and Mac-ASCs.