Figure 1
Expression of SGLT1 in human and murine endometrium. (a) qRT-PCR analysis of SLC5A1 (SGLT1) transcript levels in primary human endometrial cells (HESCs) decidualized with 0.5 M 8-br-cAMP (cAMP) and 1 μM medroxyprogesterone acetate (MPA) for 2, 4, or 6 days. Control cells remained untreated. Data were normalized to L19 (ribosomal protein 19; housekeeping gene) and expressed as fold-change relative to transcript levels of undifferentiated (control) samples. The data are means (±SEM) of 3 independent primary cultures. *Indicates P < 0.05 and ***Indicates P < 0.001 (Student’s t-test). (b) Western blot of SGLT1 in whole-cell lysates obtained from primary HESC cultures treated as indicated. GAPDH expression was used as loading control. The figures presented are cropped. Full images are in the supplementary information. (c) mSlc5a1 mRNA was expressed in uteri from Slc5a1 +/+ mice while expression was absent in Slc5a1 −/− mice. The expression of housekeeping gene mRplp2 (mouse ribosomal protein, large P2) mRNA was similar in both Slc5a1 +/+ and Slc5a1 −/− mice (n = 2). (d) Immunolocalization of mSglt1 protein in murine endometrium. Heterogeneous SGLT1 immunoreactivity was observed in the endometrial epithelium of Slc5a1 +/+ mice; in some regions the staining was apical (arrowheads), but most other cells exhibited variable intracellular, largely subapical staining. The staining was absent in the uterus of Slc5a1 −/− mice. Figures shown are representative of similar findings in 3 different animals. Bar = 20 µm.
